Research from Texas University
Recent studies on electrolyzed water indicate that anode or acidic water is most effective as disinfectants; whereas, reduced or alkaline water processed through cathode is used as safe drinking water. The present drinking water study was undertaken in two strains of autoimmune disease prone mice to establish the spontaneous disease process and longevity. Weanling MRL/lpr and NZBxNZW [B/W] F1 female mice were provided daily with (1) tap water [pH ~7.5, oxygen reduction potential (ORP)~600+] (2) electrolyzed water with pH of ~9.0 and ORP ~400- and (3) hyper-reduced water with pH~10.0 and ORP~600-. Mice were provided H2O and chow diet ad libitum and weekly body weights and spontaneous deaths were recorded. The mean survival data recorded as days for MRL/lpr mice [25 mice/group] is as follows: (1) tap water 235±25, (2) reduced water 287±40 and (3) hyper-reduced water 346±45 days [<0.05]. In the case of B/W mice [25 mice/group], (1) tap water 269±16, (2) reduced water 298±19 and (3) hyper-reduced 302±18 days. A significantly decreased (<0.05) serum lipid peroxides were observed in mice fed hyper-reduced H2O. Also, the source of water did not alter lymphocyte subsets or their response to mitogens. In summary, hyper-reduced water with pH~10.0 appears to inhibit autoimmune disease of MRL/lpr mice whereas only a modest increased lifespan was noted for B/W mice. The increased lifespan by electrolyzed H2O appears to be related to the changes in free radicals and antioxidant enzyme levels. [Supported in part by Zanix Co. and Mr. Waterman Co., Tokyo, Japan].
Water Electrolytic Reactions |
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Properties |
Characteristics |
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| Cathode Water | ||||||||||||||||
H2O ----> H+ + OH- 2H+ ----> 2e- ----> H+ |
20 ~ 30% increase of alkaline minerals in relation to the raw water. |
water is alkaline |
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| Anode Water | ||||||||||||||||
H2O ----> H+ + OH- |
20 ~ 30% increase of chlorine ions in relation to the raw water. |
water is acid |
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Experimental Design |
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| MRL/lpr NZB x NZW F1 | ||||||||||||||||
| Water | Tap water (pH 7.5) |
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Measurements |
Survival |
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Daily Analysis of Drinking H2O Given to Mice |
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| pH | ORP (mV) |
O2 content (%) | Saturation Ratio (%) | Dissolved O2 (mg/dl) | Temp (^C) | |||||||||||
Tap-Water |
7.5 |
600+ |
18 % |
80 % |
6.5 |
25.0 |
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R-Water |
9.0 |
400- |
25 % |
90 % |
7.5 |
26.0 |
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HR-Water |
10.0 |
500- |
25 % |
90 % |
7.5 |
26.5 |
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Drinking Water on Lymphocyte Subsets |
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| Treatment | CD4+ | CD8+ | Ig+ | |||||||||||||
B/W |
Tap-Water |
29±1.7 |
12.7±2.7 |
53.5±4.8 |
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R-Water |
30.8±2.4 |
13.4±2.9 |
53.0±4.6 |
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HR-Water |
32.2±3.9 |
14.4±3.9 |
50.7±6.5 |
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MRL/lpr |
Tap-Water |
23.5±0.8 |
13.7±1.1 |
32.7±3.2 |
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R-Water |
27.9±1.4* |
16.6±1.5 |
29.0±3.0 |
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*(<0.05) |
HR-Water |
30.7±3.7* |
17.2±3.1 |
29.4±2.9 |
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Effect of H2O on DNA Synthesis (CPM) in Spleen Cells of B/W Mice |
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| Control (Media) | Anti-CD3 (T cell) | LPS (B cell) | ||||||||||||||
Tap-Water |
13359±685 |
25647±5602 |
112752±2576 |
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R-Water |
17005±1061 |
26368±6571 |
109859±3572 |
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HR-Water |
14431±2519 |
32566±5939 |
117219±5092 |
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Summary: 1. Life long intake of both reduced (pH 9.0) and hyper-reduced (pH 10.0) water caused no harm to mice compared to tap water. |
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Tags: acid, alkaline water, cell, cells, disease, electrolytic water, FIR, immune, ionized water, mice, water








